SELPLG antibody (PE-FineTest®594)

Catalog No. ABIN7675468

The Rat Monoclonal anti-SELPLG antibody has been validated for FACS. It is suitable to detect SELPLG in samples from Mouse.

Quick Overview for SELPLG antibody (PE-FineTest®594) (ABIN7675468)

Target: SELPLG (Selectin P Ligand (SELPLG))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This SELPLG antibody is conjugated to PE-FineTest®594

Application: Flow Cytometry (FACS)

Clone: 4RA10

Product Details anti-SELPLG Antibody (PE-FineTest®594)

Purpose: PE-FineTest®594 Anti-Mouse CD162 Antibody

Isotype: IgG1, kappa

Application Details

Application Notes: Volume per test: 5μL. Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 μL of antibody per test (million cells in 100 μL staining volume or per 100 μL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.

Comment:

PE-FineTest®594 is designed to be excited by the blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 620 nm (e.g., a 610/20 nm bandpass filter).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0. 1 % sodium azide, 1 %BSA, pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: 4 °C

Storage Comment: 2-8°C for 12 months (Avoid repeated freeze / thaw cycles.)

Expiry Date: 12 months

Target Details for SELPLG

Target: SELPLG (Selectin P Ligand (SELPLG))

Alternative Name: CD162

Background:

Synonyms: CD162|P-selectin glycoprotein ligand 1|PSGL-1|Selectin P ligand|Selplg|PSGL1

Background: The 4RA10 antibody reacts with the N-terminal functional peptide of CD162 (P-selectin glycoprotein ligand-1, PSGL-1), encoded by the Selpl gene. PSGL-1 is expressed on the cell surface as a homodimer of approximately 230 kDa. In the mouse, Selpl mRNA is detected in most tissues, with high levels found in hematopoietic cells, brain, and adipose tissue. Flow cytometric analyses have revealed CD162 expression on bone marrow-derived mast and dendritic cells, splenic leukocytes, platelets, peripheral blood neutrophils, and neutrophil and T-cell lines. PSGL-1 is a ligand for P-selectin (CD62P) and is involved in leukocyte rolling, the migration of leukocytes into inflamed tissues, and responses to vascular injury. It is a sialomucin that must be specifically sialylated, fucosylated, and sulfated to bind P-selectin. There is also evidence that other ligands for PSGL-1 and CD62P may exist. 4RA10 mAb is reported to block the binding of mouse leukocytes to CD62P and CD62L.

Molecular Weight: 0

Gene ID: 20345

UniProt: Q62170