IL6RA antibody (AA 351-460)

Catalog No. ABIN734693

The Rabbit Polyclonal anti-IL6RA antibody has been validated for FACS, IF (cc), IF (p), ELISA, IHC (fro) and IHC (p). It is suitable to detect IL6RA in samples from Mouse, Human and Rat. There are 2+ publications available.

Quick Overview for IL6RA antibody (AA 351-460) (ABIN734693)

Target: IL6RA (Interleukin 6 Receptor, alpha (IL6RA))

Reactivity: Mouse, Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL6RA antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-IL6RA Antibody

Binding Specificity: AA 351-460

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from mouse IL-6R alpha

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-IL6RA antibody (ABIN734693)

Urushima, Fujimoto, Mishima, Ohkawara, Honda, Lee, Kawahata, Serada, Naka: "Leucine-rich alpha 2 glycoprotein promotes Th17 differentiation and collagen-induced arthritis in mice through enhancement of TGF-β-Smad2 signaling in naïve helper T cells." in: Arthritis research & therapy, Vol. 19, Issue 1, pp. 137, (2017) (PubMed).

Prystaz, Kaiser, Kovtun, Haffner-Luntzer, Fischer, Rapp, Liedert, Strauss, Waetzig, Rose-John, Ignatius: "Distinct Effects of IL-6 Classic and Trans-Signaling in Bone Fracture Healing." in: The American journal of pathology, Vol. 188, Issue 2, pp. 474-490, (2017) (PubMed).

Target Details for IL6RA

Target: IL6RA (Interleukin 6 Receptor, alpha (IL6RA))

Alternative Name: IL-6R alpha

Background:

Synonyms: Il6r, CD126, IL-6R, Interleukin-6 receptor subunit alpha, IL-6 receptor subunit alpha, IL-6R subunit alpha, IL-6R-alpha, IL-6RA, IL-6R 1, Il6ra

Background: Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis.

Gene ID: 16194

UniProt: P22272

Pathways: JAK-STAT Signaling, Autophagy, Growth Factor Binding, Cancer Immune Checkpoints