RAD52 antibody (AA 101-200)

Catalog No. ABIN735758

The Rabbit Polyclonal anti-RAD52 antibody is suitable to detect RAD52 in samples from Human. It has been validated for ELISA, IHC (p), IF (p), IF (cc) and IHC (fro).

Quick Overview for RAD52 antibody (AA 101-200) (ABIN735758)

Target: RAD52 (RAD52 Homolog (S. Cerevisiae) (RAD52))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAD52 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-RAD52 Antibody

Binding Specificity: AA 101-200

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Rad52

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for RAD52

Target: RAD52 (RAD52 Homolog (S. Cerevisiae) (RAD52))

Alternative Name: Rad52

Background:

Synonyms: DNA repair protein RAD52, DNA repair protein RAD52 homolog, RAD 52, RAD52 homolog S. cerevisiae, RAD52 homolog, Recombination protein RAD52, Rhabdomyosarcoma antigen MU RMS 40.23.

Background: DNA double-strand breaks can be generated by exposure to ionizing radiation or through endogenous cellular reactions. Double-strand breaks are generally thought to be repaired via one of two distinct mechanisms: homologous recombination (HR) or non-homologous end joining (NHEJ). Homologous recombination requires the activity of the members of the RAD52 epistasis group, which include RAD50, RAD51, RAD52, RAD54, RDH54/TID1, RAD55, RAD57, RAD59, MRE11, and XRS2. RAD52 can catalyze strand annealing reactions, and can form oligomers that attach to ends of single-stranded DNA.

Gene ID: 5893

Pathways: DNA Damage Repair