TLR4 antibody (AA 751-835)

Catalog No. ABIN736491

This anti-TLR4 antibody is a Rabbit Polyclonal antibody detecting TLR4 in WB, FACS, ELISA, IHC (p), IF (p), IF (cc) and IHC (fro). Suitable for Human, Mouse, Rat and Cow. This Primary Antibody has been cited in 13+ publications.

Quick Overview for TLR4 antibody (AA 751-835) (ABIN736491)

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Reactivity: Human, Mouse, Rat, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TLR4 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-TLR4 Antibody

Binding Specificity: AA 751-835

Cross-Reactivity: Cow, Human, Mouse, Rat

Predicted Reactivity: Dog,Sheep,Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from rat TLR4

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-TLR4 antibody (ABIN736491)

Kulhankova, Kinney, Stach, Gourronc, Grumbach, Klingelhutz, Salgado-Pabón: "The Superantigen Toxic Shock Syndrome Toxin-1 Alters Human Aortic Endothelial Cell Function." in: Infection and immunity, (2018) (PubMed).

Xu, Zheng, Liu, Li, Yang, Shang: "Glaucocalyxin B Alleviates Lipopolysaccharide-Induced Parkinson's Disease by Inhibiting TLR/NF-κB and Activating Nrf2/HO-1 Pathway." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 44, Issue 6, pp. 2091-2104, (2018) (PubMed).

Harasymowicz, Clement, Azfer, Burnett, Salter, Simpson: "Regional Differences Between Perisynovial and Infrapatellar Adipose Tissue Depots and Their Response to Class II and Class III Obesity in Patients With Osteoarthritis." in: Arthritis & rheumatology (Hoboken, N.J.), Vol. 69, Issue 7, pp. 1396-1406, (2017) (PubMed).

Wang, Li, Liu, Wang, Cao: "Inhibitory effect of Zanthoxylum bungeanum seed oil on ovalbumin‑induced lung inflammation in a murine model of asthma." in: Molecular medicine reports, Vol. 13, Issue 5, pp. 4289-302, (2017) (PubMed).

Arfian, Muflikhah, Soeyono, Sari, Tranggono, Anggorowati, Romi: "Vitamin D Attenuates Kidney Fibrosis via Reducing Fibroblast Expansion, Inflammation, and Epithelial Cell Apoptosis." in: The Kobe journal of medical sciences, Vol. 62, Issue 2, pp. E38-44, (2016) (PubMed).

Kawakita, Fujimoto, Liu, Nakano, Nakatsuka, Suzuki: "Effects of Toll-Like Receptor 4 Antagonists Against Cerebral Vasospasm After Experimental Subarachnoid Hemorrhage in Mice." in: Molecular neurobiology, (2016) (PubMed).

Wang, Shi, Lv, Xu, Li, Ge, Xiao, Geng, Liu: "Artesunate Attenuates Lipopolysaccharide-Stimulated Proinflammatory Responses by Suppressing TLR4, MyD88 Expression, and NF-?B Activation in Microglial Cells." in: Inflammation, (2015) (PubMed).

Namisaki, Noguchi, Moriya, Kitade, Aihara, Douhara, Nishimura, Takeda, Okura, Kawaratani, Takaya, Seki, Yoshiji: "Beneficial effects of combined ursodeoxycholic acid and angiotensin-II type 1 receptor blocker on hepatic fibrogenesis in a rat model of nonalcoholic steatohepatitis." in: Journal of gastroenterology, (2015) (PubMed).

Yang, Wang, Zhang, Yang, Lu, Zhang, Li, Zhang, Li: "Astragaloside IV attenuates inflammatory cytokines by inhibiting TLR4/NF-?B signaling pathway in isoproterenol-induced myocardial hypertrophy." in: Journal of ethnopharmacology, Vol. 150, Issue 3, pp. 1062-70, (2014) (PubMed).

Chen, Yu, Fan, Bian, Ma, Lu, Jin: "Sargassum fusiforme polysaccharide activates nuclear factor kappa-B (NF-?B) and induces cytokine production via Toll-like receptors." in: Carbohydrate polymers, Vol. 105, pp. 113-20, (2014) (PubMed).

Xing, Zheng, Zhang, Wang, Xing: "Relationship between toll-like receptor 4 levels in aorta and severity of atherosclerosis." in: The Journal of international medical research, Vol. 42, Issue 4, pp. 958-965, (2014) (PubMed).

Takanashi, Tomosada, Villena, Murata, Takahashi, Chiba, Tohno, Shimazu, Aso, Suda, Ikegami, Itoh, Kawai, Saito, Alvarez, Kitazawa: "Advanced application of bovine intestinal epithelial cell line for evaluating regulatory effect of lactobacilli against heat-killed enterotoxigenic Escherichia coli-mediated inflammation." in: BMC microbiology, Vol. 13, pp. 54, (2013) (PubMed).

Tang, Zhou, Wang, Zhang, Wang, Li, Huang: "Expression of TRAF6 and pro-inflammatory cytokines through activation of TLR2, TLR4, NOD1, and NOD2 in human periodontal ligament fibroblasts." in: Archives of oral biology, Vol. 56, Issue 10, pp. 1064-72, (2011) (PubMed).

Target Details for TLR4

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Alternative Name: TLR4

Background:

Synonyms: Lps, Ly87, Ran/M1, Rasl2-8, Toll-like receptor 4, CD284, Tlr4

Background: Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by free fatty acids, such as palmitate. In complex with TLR6, promotes sterile inflammation in monocytes/macrophages in response to oxidized low-density lipoprotein (oxLDL) or amyloid-beta 42. In this context, the initial signal is provided by oxLDL- or amyloid-beta 42-binding to CD36. This event induces the formation of a heterodimer of TLR4 and TLR6, which is rapidly internalized and triggers inflammatory response, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion.

Gene ID: 21898

UniProt: Q9QX05

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome, S100 Proteins