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K-RAS antibody (AA 25-130)

Name: K-RAS antibody (AA 25-130)
SKU: ABIN736671
Price: 384.62 USD
Availability: InStock

This anti-K-RAS antibody is a Rabbit Polyclonal antibody detecting K-RAS in WB, ELISA, FACS, IF (cc), IF (p), IHC (p) and IHC (fro). Suitable for Human, Rat and Mouse.

Formalin-fixed and paraffin embedded: human endometrium tissue labeled with Anti-KRAS Polyclonal Antibody (ABIN736671), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining (K-RAS antibody (AA 25-130))

IHC

Paraformaldehyde-fixed, paraffin embedded Mouse Kidney tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30min, Antibody incubation with KRAS Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining (K-RAS antibody (AA 25-130))

IHC (p)

Paraformaldehyde-fixed, paraffin embedded mouse kidney, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30min, Antibody incubation with KRAS Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary (Goat Anti-rabbit IgG/Bio) for 20minutes at 37°C, followed by a conjugated streptavidin at 1:500 for 40 minutes and DAPI staining of the nuclei. (K-RAS antibody (AA 25-130))

IF (p)

3 images

Catalog No. ABIN736671

Quick Overview for K-RAS antibody (AA 25-130) (ABIN736671)

Target

See all K-RAS (KRAS) Antibodies

K-RAS (KRAS) (GTPase Kras (KRAS))

Reactivity

Human, Rat, Mouse

Host

Rabbit

Clonality

Polyclonal

Conjugate

This K-RAS antibody is un-conjugated

Application

Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Discover our top product KRAS Primary Antibody

Binding Specificity
15

10

8

7

6

6

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 25-130

Cross-Reactivity

Human, Mouse, Rat

Purification

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from human K-ras

Isotype

IgG
anti-GTPase Kras (KRAS) (AA 1-188) antibody
KRAS Reactivity: Human WB, ELISA, IF Host: Mouse Monoclonal 3B10-2F2 unconjugated

anti-GTPase Kras (KRAS) (AA 16-125) antibody
KRAS Reactivity: Human WB, ELISA Host: Mouse Monoclonal 4F3 unconjugated

anti-GTPase Kras (KRAS) (AA 146-174) antibody
KRAS Reactivity: Human, Rat, Mouse WB, ELISA, IF, FACS Host: Rabbit Polyclonal unconjugated

anti-GTPase Kras (KRAS) (AA 2-168) antibody
KRAS Reactivity: Human WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-GTPase Kras (KRAS) (pSer89) antibody
KRAS Reactivity: Human WB, IHC Host: Rabbit Polyclonal unconjugated

anti-GTPase Kras (KRAS) (N-Term) antibody
KRAS Reactivity: Human, Rat, Mouse, Dog, Zebrafish (Danio rerio), Rabbit, Horse, Guinea Pig, Cow, Drosophila melanogaster, Pig, Bat, Chicken, Xenopus laevis, Hamster, Monkey WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-GTPase Kras (KRAS) (N-Term) antibody
KRAS Reactivity: Human, Rat, Mouse, Dog, Zebrafish (Danio rerio), Rabbit, Horse, Guinea Pig, Cow, Saccharomyces cerevisiae WB Host: Rabbit Polyclonal unconjugated

anti-GTPase Kras (KRAS) (AA 1-189) antibody
KRAS Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-GTPase Kras (KRAS) (C-Term) antibody
KRAS Reactivity: Human, Rat, Mouse WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated

anti-GTPase Kras (KRAS) (AA 30-188) antibody
KRAS Reactivity: Human WB, IF Host: Rabbit Polyclonal unconjugated

Application Notes

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Target

K-RAS (KRAS) (GTPase Kras (KRAS))

Alternative Name

KRAS

Background

Synonyms: NS, NS3, CFC2, KRAS1, KRAS2, RASK2, KI-RAS, C-K-RAS, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, GTPase KRas, K-Ras 2, c-Ki-ras, KRAS
Background: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the regulation of cell proliferation (PubMed:23698361, PubMed:22711838).

Gene ID

3845

UniProt

P01116