Recombinant Topoisomerase II alpha antibody (AA 650-700)

Catalog No. ABIN7795794

The Rabbit Monoclonal anti-Topoisomerase II alpha antibody is suitable to detect Topoisomerase II alpha in samples from Human and Mouse. It has been validated for WB, IHC and ICC.

Quick Overview for Recombinant Topoisomerase II alpha antibody (AA 650-700) (ABIN7795794)

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Topoisomerase II alpha antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Clone: BLR358N

Product Details anti-Topoisomerase II alpha Antibody

Binding Specificity: AA 650-700

Purpose: Rabbit anti-Topo II Alpha Recombinant Monoclonal Antibody [BLR358N]

Purification: Purified

Immunogen: between 650 and 700

Isotype: IgG

Application Details

Application Notes: ICC,1100 to 1500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.,IHC,1100 to 1500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.,WB,11,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: Phosphate Buffered Saline (PBS) with 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2 - 8 °C

Expiry Date: 12 months

Target Details for Topoisomerase II alpha

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Alternative Name: Topo II Alpha

Background: Topoisomerases regulate topological states of DNA and are divided into type I and type II enzymes. Type II topoisomerases make double stranded breaks in DNA and allow the passage of another duplex DNA across the break to achieve unknotting of entangled DNA. Topo II Alpha is one of two described isoforms that are likely products of a gene duplication event. TopoII Alpha is also described to be involved in chromosome condensation, chromatid separation, and the relief of supercoil stress.

Gene ID: 7153

UniProt: P11388

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases