Recombinant PDX1 antibody

Catalog No. ABIN7799731

The Rabbit Monoclonal anti-PDX1 antibody (Clone 24GB1380) (ABIN7799731) specifically detects PDX1 in WB, ICC, IF (p) and FACS. The antibody is reactive with Human samples.

Quick Overview for Recombinant PDX1 antibody (ABIN7799731)

Target: PDX1 (Pancreatic and Duodenal Homeobox 1 (PDX1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PDX1 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Flow Cytometry (FACS)

Grade: KD Validated

Clone: 24GB1380

Product Details anti-PDX1 Antibody

Expression System: HEK-293 Cells

Purpose: KD-Validated Pancreatic And Duodenal Homeobox 1 Recombinant Rabbit mAb

Cross-Reactivity: Rat

Purification: Affinity Purified

Immunogen: A synthesized peptide derived from human PDX1

Isotype: IgG

Application Details

Application Notes: Western Blotting (WB): 1:1,000-1:5,000, Flow Cytometry (FCM): 1:200-1:2,000, Immunocytochemistry (ICC): 1:100-1:1,000, Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200

Comment:

We assess the antibodies’ specificity and selectivity using knockdown techniques. Our validation approach is straightforward: if an antibody identifies a signal in wild-type (WT) cells, but this signal is absent or noticeably reduced in gene-silenced (KD) cells, we consider the antibody to be highly specific.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4, with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C for one year.

Expiry Date: 12 months

Target Details for PDX1

Target: PDX1 (Pancreatic and Duodenal Homeobox 1 (PDX1))

Alternative Name: Pancreatic And Duodenal Homeobox 1

Molecular Weight: 31 kDa

Gene ID: 3651

UniProt: P52945

Pathways: Nuclear Receptor Transcription Pathway, Positive Regulation of Peptide Hormone Secretion, Steroid Hormone Mediated Signaling Pathway, Hormone Transport, Carbohydrate Homeostasis, Chromatin Binding, Maintenance of Protein Location