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Recombinant Vimentin antibody (pSer56)

The Rabbit Monoclonal anti-Vimentin antibody has been validated for WB, FACS and IF (p). It is suitable to detect Vimentin in samples from Human.
Catalog No. ABIN7800834

Quick Overview for Recombinant Vimentin antibody (pSer56) (ABIN7800834)

Target

See all Vimentin (VIM) Antibodies
Vimentin (VIM)

Antibody Type

Recombinant Antibody

Reactivity

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Human

Host

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Rabbit

Clonality

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  • 179
Monoclonal

Conjugate

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This Vimentin antibody is un-conjugated

Application

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Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Grade

KD Validated

Clone

25GB2035
  • Binding Specificity

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    pSer56

    Expression System

    HEK-293 Cells

    Purpose

    KD-Validated Phospho-Vimentin (Ser56) Recombinant Rabbit mAb

    Purification

    Affinity Purified

    Immunogen

    A synthesized peptide derived from human Phospho-Vimentin (Ser56)

    Isotype

    IgG
  • Application Notes

    Western Blotting (WB): 1:1,000-1:5,000, Flow Cytometry (FCM): 1:200-1:2,000, Immunohistochemistry-Paraffin (IHC-P): 1:50-1:100

    Comment

    We assess the antibodies’ specificity and selectivity using knockdown techniques. Our validation approach is straightforward: if an antibody identifies a signal in wild-type (WT) cells, but this signal is absent or noticeably reduced in gene-silenced (KD) cells, we consider the antibody to be highly specific.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Buffer

    PBS, pH 7.4, with 0.02 % sodium azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    -20 °C

    Storage Comment

    Store at -20 °C for one year.

    Expiry Date

    12 months
  • Target

    Vimentin (VIM)

    Alternative Name

    Vimentin

    Molecular Weight

    54 kDa

    Gene ID

    7431

    UniProt

    P08670

    Pathways

    Caspase Cascade in Apoptosis
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