Vimentin antibody

Catalog No. ABIN7800836

This Mouse Monoclonal antibody specifically detects Vimentin in WB, FACS and IF (p). It exhibits reactivity toward Human.

Quick Overview for Vimentin antibody (ABIN7800836)

Target: Vimentin (VIM)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Vimentin antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Grade: KD Validated

Clone: 25GB2725

Product Details anti-Vimentin Antibody

Purpose: KD-Validated Vimentin Mouse mAb

Cross-Reactivity: Mouse, Rat

Purification: Affinity Purified

Immunogen: Recombinant protein of human Vimentin

Isotype: IgM kappa

Application Details

Application Notes: Western Blotting (WB): 1:1,000-1:5,000, Flow Cytometry (FCM): 1:200-1:2,000, Immunohistochemistry-Paraffin (IHC-P): 1:50-1:100

Comment:

We assess the antibodies’ specificity and selectivity using knockdown techniques. Our validation approach is straightforward: if an antibody identifies a signal in wild-type (WT) cells, but this signal is absent or noticeably reduced in gene-silenced (KD) cells, we consider the antibody to be highly specific.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4, with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C for one year.

Expiry Date: 12 months

Target Details for Vimentin

Target: Vimentin (VIM)

Alternative Name: Vimentin

Molecular Weight: 54 kDa

Gene ID: 7431

UniProt: P08670

Pathways: Caspase Cascade in Apoptosis