Tyrosine Hydroxylase antibody

Catalog No. ABIN7822945

This Mouse Monoclonal antibody specifically detects Tyrosine Hydroxylase in WB, IHC, ICC, IP and ELISA. It exhibits reactivity toward Human.

Quick Overview for Tyrosine Hydroxylase antibody (ABIN7822945)

Target: Tyrosine Hydroxylase (TH)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Tyrosine Hydroxylase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP), ELISA

Clone: LNC1

Product Details anti-Tyrosine Hydroxylase Antibody

Purpose: Anti-Tyrosine Hydroxylase Antibody (Purified)

Specificity: Recognizes an epitope outside of the regulatory N-terminus. Recognizes a protein of approximately 59-61 kDa by Western blot. No off-targets reported for the following on Western Blots: purified dopamine-beta-hydroxylase, phenylalanine hydroxylase, trytophan hydroxylase, dehydropteridine reductase, sepiapterin reductase or phenethanolamine-N-methyl transferase (PNMT). Identifies a single ~60 kDa band on Western Blots of HeLa cells transfected to express human tyrosine hydroxylase.

Cross-Reactivity: Chicken, Frog, Lizard, Microtus arvalis (Common vole) (Field vole), Monkey, Mouse, Rat, Zebrafish (Danio rerio)

Characteristics: Our Anti-Tyrosine Hydroxylase (TH) mouse monoclonal primary antibody is produced in-house from hybridoma clone LNC1. It detects chicken, frog, human, lizard, monkey, mouse, rat, vole, and zebrafish Tyrosine Hydroxylase (TH), and is purified by protein a chromatography. It is great for use in WB, IHC, ICC, IP, ELISA.

Purification: Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Purity: Purified mAbs are >90 % specific antibody.

Immunogen: Tyrosine Hydroxylase purified from PC12 cells

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 10 mM Tris, 50 mM Sodium Chloride, 0.065 % Sodium Azide 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: For maximum recovery of product, centrifuge the vial prior to removing the cap.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C.

Expiry Date: 24 months

Target Details for Tyrosine Hydroxylase

Target: Tyrosine Hydroxylase (TH)

Alternative Name: Tyrosine Hydroxylase

Background:

Synonyms: Tyrosine 3-monooxygenase

Description Logic: Our Anti-Tyrosine Hydroxylase (TH) mouse monoclonal primary antibody is produced from hybridoma clone LNC1. It detects chicken, frog, human, lizard, monkey, mouse, rat, vole, and zebrafish Tyrosine Hydroxylase (TH), and is purified by protein a chromatography. It is great for use in WB, IHC, ICC, IP, ELISA.

Gene Name Alternatives: TH TYH EC 1.14.16.2

Molecular Weight: 59-63 kDa

UniProt: P07101

Pathways: Dopaminergic Neurogenesis, Response to Water Deprivation, Sensory Perception of Sound, Carbohydrate Homeostasis, Feeding Behaviour