DNMT3A antibody (AA 1-284)

Catalog No. ABIN7825343

This anti-DNMT3A antibody is a Rabbit Polyclonal antibody detecting DNMT3A in WB, ELISA, IHC, IF, FACS, IP and ICC. Suitable for Human, Mouse and Rat.

Quick Overview for DNMT3A antibody (AA 1-284) (ABIN7825343)

Target: DNMT3A (DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DNMT3A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-DNMT3A Antibody

Binding Specificity: AA 1-284

Purpose: Anti-DNMT3A Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-DNMT3A Antibody catalog # A00136-2. Tested in WB, IHC, ICC, IF, IP, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human DNMT3A recombinant protein (Position: M1-Y284).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Immunoprecipitation, 0.5-2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for DNMT3A

Target: DNMT3A (DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A))

Alternative Name: DNMT3A

Background: Methylation of DNA at cytosine residues in mammalian cells is a heritable, epigenetic modification that is critical for proper regulation of gene expression, genomic imprinting and development. Three families of mammalian DNA methyltransferases have been identified: DNMT1, DNMT2 and DNMT3. DNMT1 is constitutively expressed in proliferating cells and functions as a maintenance methyltransferase, transferring proper methylation patterns to newly synthesized DNA during replication.

Molecular Weight: 120-130 kDa

UniProt: Q9Y6K1