GCNT2 antibody (AA 27-397)

Catalog No. ABIN7825405

The Rabbit Polyclonal anti-GCNT2 antibody is suitable to detect GCNT2 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC, FACS, ICC and IF.

Quick Overview for GCNT2 antibody (AA 27-397) (ABIN7825405)

Target: GCNT2 (Glucosaminyl (N-Acetyl) Transferase 2, I-Branching Enzyme (I Blood Group) (GCNT2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GCNT2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-GCNT2 Antibody

Binding Specificity: AA 27-397

Purpose: Anti-GCNT2 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-GCNT2 Antibody catalog # A07194. Tested in WB, IHC, ICC/IF, IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human GCNT2 recombinant protein (Position: E27-Q397). Human GCNT2 shares 71.7% amino acid (aa) sequence identity with mouse GCNT2.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for GCNT2

Target: GCNT2 (Glucosaminyl (N-Acetyl) Transferase 2, I-Branching Enzyme (I Blood Group) (GCNT2))

Alternative Name: GCNT2

Background: This gene encodes the enzyme responsible for formation of the blood group I antigen. The i and I antigens are distinguished by linear and branched poly-N-acetyllactosaminoglycans, respectively. The encoded protein is the I-branching enzyme, a beta-1,6-N-acetylglucosaminyltransferase responsible for the conversion of fetal i antigen to adult I antigen in erythrocytes during embryonic development. Mutations in this gene have been associated with adult i blood group phenotype. Alternatively spliced transcript variants encoding different isoforms have been described.

Molecular Weight: 46 kDa

Gene ID: 2651

UniProt: Q8N0V5

Pathways: Glycosaminoglycan Metabolic Process