PPP1R12A antibody (AA 1-40)

Catalog No. ABIN7825566

This Rabbit Polyclonal antibody specifically detects PPP1R12A in WB and IHC. It exhibits reactivity toward Zebrafish (Danio rerio).

Quick Overview for PPP1R12A antibody (AA 1-40) (ABIN7825566)

Target: PPP1R12A (Myosin Phosphatase, Target Subunit 1 (PPP1R12A))

Reactivity: Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPP1R12A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-PPP1R12A Antibody

Binding Specificity: AA 1-40

Purpose: Anti-Zebrafish MYPT1/PPP1R12A Antibody

Characteristics: Anti-Zebrafish MYPT1/PPP1R12A Antibody catalog # AZQ6DRG7. Tested in WB, IHC applications. This antibody reacts with Zebrafish. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived zebrafish MYPT1/PPP1R12A recombinant protein (Position: M1-D40).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Zebrafish
Immunohistochemistry, 2-5 μg/mL, Zebrafish

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for PPP1R12A

Target: PPP1R12A (Myosin Phosphatase, Target Subunit 1 (PPP1R12A))

Alternative Name: PPP1R12A

Background: PPP1R12A (Protein phosphatase 1 regulatory subunit 12A), also called MYPT1 (Myosin phosphatase target subunit 1), is an enzyme that in humans is encoded by the PPP1R12A gene. PPP1R12A is one of the subunits of myosin phosphatase. Sequencing analysis showed that human PPP1R12A contains 1,030 amino acids with a calculated molecular mass of approximately 115 kD. The PPP1R12A gene is mapped on 12q21.2-q21.3. PPP1R12A is the protein that regulates PP1 function in smooth muscle relaxation. The cellular MYPT1-PP1-delta -specific inhibitor CPI17 caused a loss of merlin function characterized by merlin phosphorylation, Ras activation, and transformation. Jin et al. concluded that PPP1R12A and its substrate merlin are part of a previously undescribed tumor suppressor cascade that can be hindered in two ways, by mutation of the NF2 gene and by upregulation of the oncoprotein CPI17.

Molecular Weight: 140 kDa

Pathways: M Phase