NEFM antibody (AA 125-916)

Catalog No. ABIN7825592

This anti-NEFM antibody is a Rabbit Polyclonal antibody detecting NEFM in WB, IHC, IF, ELISA, IP and FACS. Suitable for Human, Rat and Mouse.

Quick Overview for NEFM antibody (AA 125-916) (ABIN7825592)

Target: NEFM (Neurofilament Triplet M Protein (NEFM))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEFM antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS)

Product Details anti-NEFM Antibody

Binding Specificity: AA 125-916

Purpose: Anti-NF-M/NEFM Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-NF-M/NEFM Antibody catalog # A06821-2. Tested in WB, IHC, IF, IP, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NF-M/NEFM recombinant protein (Position: Q125-D916). Human NF-M/NEFMshares 72.3% and 72% amino acid (aa) sequence identity with mouse and rat NF-M/NEFM, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Mouse, Rat
Immunoprecipitation, 0.5-2 μg/mL, Human
Immunofluorescence, 5 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for NEFM

Target: NEFM (Neurofilament Triplet M Protein (NEFM))

Alternative Name: NEFM

Background: Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Molecular Weight: 160 kDa

Gene ID: 4741

UniProt: P07197

Pathways: Brown Fat Cell Differentiation