PAM antibody (AA 69-914)

Catalog No. ABIN7825666

The Rabbit Polyclonal anti-PAM antibody has been validated for WB, ELISA, IHC and FACS. It is suitable to detect PAM in samples from Human, Mouse and Rat.

Quick Overview for PAM antibody (AA 69-914) (ABIN7825666)

Target: PAM (Peptidylglycine alpha-Amidating Monooxygenase (PAM))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAM antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-PAM Antibody

Binding Specificity: AA 69-914

Purpose: Anti-PAM Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-PAM Antibody catalog # A00332-2. Tested in WB, IHC, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PAM recombinant protein (Position: K69-R914). Human PAM shares 97.4% amino acid (aa) sequence identity with mouse PAM.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for PAM

Target: PAM (Peptidylglycine alpha-Amidating Monooxygenase (PAM))

Alternative Name: PAM

Background: This gene encodes a multifunctional protein. The encoded preproprotein is proteolytically processed to generate the mature enzyme. This enzyme includes two domains with distinct catalytic activities, a peptidylglycine alpha-hydroxylating monooxygenase (PHM) domain and a peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PAL) domain. These catalytic domains work sequentially to catalyze the conversion of neuroendocrine peptides to active alpha-amidated products. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.

Molecular Weight: 120 kDa

Gene ID: 5066

UniProt: P19021