PIF1 antibody (AA 31-618)

Catalog No. ABIN7825692

This Rabbit Polyclonal antibody specifically detects PIF1 in ELISA, WB, IHC and FACS. It exhibits reactivity toward Human.

Quick Overview for PIF1 antibody (AA 31-618) (ABIN7825692)

Target: PIF1 (PIF1 5'-To-3' DNA Helicase (PIF1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIF1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-PIF1 Antibody

Binding Specificity: AA 31-618

Purpose: Anti-PIF1 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human PIF1 shares 87.6%,87.8% amino acid (aa) sequence identity with mouse,rat PIF1,respectively.

Characteristics: Anti-PIF1 Antibody. Tested in WB, IHC, Flow Cytometry, ELISA applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PIF1 recombinant protein (Position: Q31-R618). Human PIF1 shares 87.6% and 87.8% amino acid (aa) sequence identity with mouse and rat PIF1, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PIF1

Target: PIF1 (PIF1 5'-To-3' DNA Helicase (PIF1))

Alternative Name: PIF1

Background:

Background: This gene encodes a DNA-dependent adenosine triphosphate (ATP)-metabolizing enzyme that functions as a 5' to 3' DNA helicase. The encoded protein can resolve G-quadruplex structures and RNA-DNA hybrids at the ends of chromosomes. It also prevents telomere elongation by inhibiting the actions of telomerase. Alternative splicing and the use of alternative start codons results in multiple isoforms that are differentially localized to either the mitochondria or the nucleus.

Gene Full Name: PIF1 5'-to-3' DNA helicase

Molecular Weight: 77 kDa

Gene ID: 80119

UniProt: Q9H611