Recombinant Cyclin E1 antibody

Catalog No. ABIN7826111

The Rabbit Monoclonal anti-Cyclin E1 antibody has been validated for IHC. It is suitable to detect Cyclin E1 in samples from Human.

Quick Overview for Recombinant Cyclin E1 antibody (ABIN7826111)

Target: Cyclin E1 (CCNE1)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Cyclin E1 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: 898R

Product Details anti-Cyclin E1 Antibody

Purpose: Cyclin E1 antibody (898R)

Immunogen: Recombinant human Cyclin E1 protein fragment

Isotype: IgG

Application Details

Application Notes: IHC 1:50-1:100

Comment:

Positive Control: Placenta: A strong Cyclin E1 staining should be seen in most cytotrophoblast cells.

Product Description/Background: Cyclin E1 antibody validated for immunohistochemistry on 76 different Normal Tissues

Negative Control: Normal kidney: Cyclin E1 staining should be largely absent in tubular and glomerular cells.

Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:50 at 37 °C for 60 minutes.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non- hazardous. No MSD required.

Expiry Date: 24 months

Target Details for Cyclin E1

Target: Cyclin E1 (CCNE1)

Alternative Name: Cyclin E1

Background: Gene Alias: CCNE1, Cyclin E1, G1/S-specific cyclin-E1, G1/S-specific cyclin-E1

UniProt: P24864

Pathways: Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Mitotic G1-G1/S Phases