Recombinant HMGB1 antibody (N-Term)

Catalog No. ABIN7826123

The Rabbit Monoclonal anti-HMGB1 antibody has been validated for IHC. It is suitable to detect HMGB1 in samples from Human.

Quick Overview for Recombinant HMGB1 antibody (N-Term) (ABIN7826123)

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This HMGB1 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: HMV317

Product Details anti-HMGB1 Antibody

Binding Specificity: N-Term

Purpose: HMGB1 antibody (HMV317)

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human HMGB1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Colon: A strong HMGB1 positivity should be seen in all cell types.

Product Description/Background: HMGB1 antibody validated for immunohistochemistry on 76 different Normal Tissues

Negative Control: Testis: Spermatids and more mature spermatocytes should be HMGB1 negative.

Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non- hazardous. No MSD required.

Expiry Date: 24 months

Target Details for HMGB1

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Alternative Name: HMGB1

Background: Gene Alias: high mobility group box 1,HMG-1,HMG1,HMG3,SBP-1

UniProt: P09429

Pathways: p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome