Recombinant Cyclin D1 antibody (pThr286)

Catalog No. ABIN7828139

The Rabbit Monoclonal anti-Cyclin D1 antibody is suitable to detect Cyclin D1 in samples from Human, Mouse and Rat. It has been validated for WB, IHC and FACS.

Quick Overview for Recombinant Cyclin D1 antibody (pThr286) (ABIN7828139)

Target: Cyclin D1 (CCND1)

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Cyclin D1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: A202

Product Details anti-Cyclin D1 Antibody

Binding Specificity: pThr286

Purpose: Recombinant Phospho-Cyclin D1 (Thr286) Monoclonal Antibody

Purification: Protein A purified

Immunogen: Recombinant human Phospho-Cyclin D1 (Thr286) fragment

Isotype: IgG, kappa

Application Details

Application Notes: WB 1:500-1:2000,IHC 1:50-1:100,FCM 1:50

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 % protein protectant.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Expiry Date: 12 months

Target Details for Cyclin D1

Target: Cyclin D1 (CCND1)

Alternative Name: Cyclin D1

Background: Cyl,PRAD,U21B,CCND,B cell leukemia,G1/S specific cyclin D,Cyclin D,BCL,BCL1,D11S287E,PRAD1,U21B31,CCND1,Cyclin D1,B cell leukemia 1,BCL 1,Cd1,Cyl 1,G1/S specific cyclin D1,BCL1,D11S287E,PRAD1,U21B31,CCND1,Cyclin D1,cyclin D1,Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G1/S transition. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner. Cat.No. Product Name Clone No. IF:{{item.impact}} Journal:{{item.journal}} ({{item.year}}) DOI:{{item.doi}} Reactivity:{{item.species}} Sample Type:{{item.sample_type}} Previous {{ page }} Next Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}} Previous {{ page }} Next [

Molecular Weight:

Calculated MW: 34 kDa

Observed MW: 34 kDa

UniProt: P24385

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, ER-Nucleus Signaling