Recombinant Glypican 3 antibody

Catalog No. ABIN7828240

This Rabbit Monoclonal antibody specifically detects Glypican 3 in WB, IF and IHC. It exhibits reactivity toward Human.

Quick Overview for Recombinant Glypican 3 antibody (ABIN7828240)

Target: Glypican 3 (GPC3)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Glypican 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Clone: A577

Product Details anti-Glypican 3 Antibody

Purpose: Recombinant Glypican-3 Monoclonal Antibody

Purification: Protein A purified

Immunogen: Recombinant human Glypican-3 fragment

Isotype: IgG, kappa

Application Details

Application Notes: WB 1:2000-1:10000,IHC 1:100-1:500,IF 1:50

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 % protein protectant.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Expiry Date: 12 months

Target Details for Glypican 3

Target: Glypican 3 (GPC3)

Alternative Name: Glypican-3

Background: MXR,GPC,UNQ,PRO,CDw,ADAM,Protein FOAP,Secreted glypican,Glypican proteoglycan,Sialic acid-binding Ig-like lectin,C20orf,OCI,GPC3,DGSX,GTR2-2,MXR7,OCI-5,SDYS,SGB,SGBS,SGBS1,glypican-3,CDw329,Glypican 3,Glypican proteoglycan 3,Glypican-3 [Precursor],GTR2 2,Heparan sulphate proteoglycan,Intestinal protein OCI 5,Intestinal protein OCI-5,OCI 5,Protein FOAP-9,Secreted glypican-3,Sialic acid-binding Ig-like lectin 9,SIGLEC9,Siglec-9,ADAM33,C20orf153,UNQ873,PRO1891,OCI5,Glypican 3 belongs to Glypicans (GPCs), a family of glycosylphosphatidylinositol (GPI)-anchored heparan sulphate proteoglycans (HSPGs) that may play a role in the control of cell division and growth regulation. In mammals, there are six GPCs (GPC1 to GPC6), all of which have a similar core-protein size of approx. 60 kDa and the clustering of glycosaminoglycan attachment site near the C-terminus. They are tethered to the cell surface by GPI linkages, which can be cleaved by endogenous phospholipases, thus releasing the protein. Glypican 3 (GPC3) is highly expressed in many tissues during development and plays an important role in the regulation of embryonic growth. Loss-of-function mutations of GPC3 result in the Simpson-Golabi-Behmel overgrowth syndrome (SGBS), and Gpc-3 null mice display developmental overgrowth. In hepatocellular carcinoma (HCC), the overexpression of glypican 3 has been demonstrated to be a reliable diagnostic indicator. Cat.No. Product Name Clone No. IF:{{item.impact}} Journal:{{item.journal}} ({{item.year}}) DOI:{{item.doi}} Reactivity:{{item.species}} Sample Type:{{item.sample_type}} Previous {{ page }} Next Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}} Previous {{ page }} Next [

Molecular Weight:

Calculated MW: 70, 40 kDa

Observed MW: 15, 70 kDa The actual band is not consistent with the expectation.

UniProt: P51654

Pathways: Glycosaminoglycan Metabolic Process