Recombinant HMGB1 antibody

Catalog No. ABIN7828259

The Rabbit Monoclonal anti-HMGB1 antibody is suitable to detect HMGB1 in samples from Human, Mouse and Rat. It has been validated for WB, IHC, FACS and IP.

Quick Overview for Recombinant HMGB1 antibody (ABIN7828259)

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This HMGB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: A253

Product Details anti-HMGB1 Antibody

Purpose: Recombinant HMGB1 Monoclonal Antibody

Purification: Protein A purified

Immunogen: Recombinant human HMGB1 fragment

Isotype: IgG, kappa

Application Details

Application Notes: WB 1:500-1:2000,IHC 1:200-1:1000,FCM 1:50-1:100,IP 1:25

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 % protein protectant.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Expiry Date: 12 months

Target Details for HMGB1

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Alternative Name: HMGB1

Background: SBP,HMGB,High Mobility Group Protein,High mobility group protein B,HMG,HMG-1,HMG1,HMG3,SBP-1,HMGB1,Amphoterin,High Mobility Group Protein 1,High mobility group protein B1,High mobility group protein 1 (HMG-1),High mobility group protein 1,high mobility group protein B1,high-mobility group box 1,HMG1,HMG-1,HMG1DKFZp686A04236,HMG3,HMGB1,SBP-1,Chromosomal protein,DKFZp686A04236,High mobility group 1,High mobility group box 1,high-mobility group (nonhistone chromosomal) protein 1,HMGB 1,nonhistone,NONHISTONE CHROMOSOMAL PROTEIN HMG1,SBP 1,Sulfoglucuronyl carbohydrate binding protein,Multifunctional redox sensitive protein with various roles in different cellular compartments. In the nucleus is one of the major chromatin-associated non-histone proteins and acts as a DNA chaperone involved in replication, transcription, chromatin remodeling, V(D)J recombination, DNA repair and genome stability. Proposed to be an universal biosensor for nucleic acids. Promotes host inflammatory response to sterile and infectious signals and is involved in the coordination and integration of innate and adaptive immune responses. In the cytoplasm functions as sensor and/or chaperone for immunogenic nucleic acids implicating the activation of TLR9-mediated immune responses, and mediates autophagy. Acts as danger associated molecular pattern (DAMP) molecule that amplifies immune responses during tissue injury. Cat.No. Product Name Clone No. IF:{{item.impact}} Journal:{{item.journal}} ({{item.year}}) DOI:{{item.doi}} Reactivity:{{item.species}} Sample Type:{{item.sample_type}} Previous {{ page }} Next Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}} Previous {{ page }} Next [

Molecular Weight:

Calculated MW: 25 kDa

Observed MW: 25 kDa

UniProt: P09429

Pathways: p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome