Recombinant Histone H2B antibody

Catalog No. ABIN7828263

The Rabbit Monoclonal anti-Histone H2B antibody (Clone A831) (ABIN7828263) specifically detects Histone H2B in WB and IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Recombinant Histone H2B antibody (ABIN7828263)

Target: Histone H2B

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Histone H2B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: A831

Product Details anti-Histone H2B Antibody

Purpose: Recombinant Histone H2B (Ubiquityl Lys120) Monoclonal Antibody

Purification: Protein A purified

Immunogen: Ubiquitinated human histone H2B (Lys120) peptide

Isotype: IgG, kappa

Application Details

Application Notes: WB 1:500-1:2000,IHC 1:200-1:1000

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 % protein protectant.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Expiry Date: 12 months

Target Details for Histone H2B

Target: Histone H2B

Background: HIST1H2BG,H2B.1A,H2B/a,H2BFA,H2BFL,HIST1H2BC,H2BFH,HIST1H2BE,H2BFG,HIST1H2BF,H2BFK,HIST1H2BI,H2BC4,H2BC6,H2BC7,H2BC8,H2BC10,Ubiquitin (Ub) is a highly conserved 76-amino acid protein that plays a critical role in regulating a wide range of cellular processes. Through a three-step process involving Ub-activating (E1), Ub-conjugating (E2), and Ub-ligating (E3) enzymes, ubiquitin covalently attaches to target proteins and marks them for proteasomal degradation, alters protein-protein interactions, modulates membrane protein trafficking, and controls the activity of many signal transduction pathways. Mono-ubiquitination of Histone H2B at Lys120 (H2BK120ub) serves signaling purposes and is reversible by ubiquitin-specific proteases (USPs). The enzymes responsible for H2BK120ub are Rad6 E2 proteins (HR6A and HR6B in human) and Bre1 E3 ligase (RNF20 and RNF40 in human). This modification triggers a ""trans-histone"" modification cascade, resulting in trimethylation of Lys4 and Lys79 in histone H3, which promotes gene activity. Cat.No. Product Name Clone No. IF:{{item.impact}} Journal:{{item.journal}} ({{item.year}}) DOI:{{item.doi}} Reactivity:{{item.species}} Sample Type:{{item.sample_type}} Previous {{ page }} Next Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}} Previous {{ page }} Next [

Molecular Weight:

Calculated MW: 23 kDa

Observed MW: 23 kDa

UniProt: P62807