Recombinant Histone H2B antibody (H2BK46ac)

Catalog No. ABIN7828264

This Rabbit Monoclonal antibody specifically detects Histone H2B in WB, IF and ChIP. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Recombinant Histone H2B antibody (H2BK46ac) (ABIN7828264)

Target: Histone H2B

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Histone H2B antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP)

Clone: A101

Product Details anti-Histone H2B Antibody

Binding Specificity: H2BK46ac

Purpose: Recombinant Histone H2B (Acetyl Lys46) Monoclonal Antibody

Purification: Protein A purified

Immunogen: Acetylated human histone H2B (Lys46) peptide

Isotype: IgG, kappa

Application Details

Application Notes: WB 1:500-1:2000,ChIP 6 μg/5x106 cells,IF 1:200

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 % protein protectant.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Expiry Date: 12 months

Target Details for Histone H2B

Target: Histone H2B

Background: HIST1H2BG,H2B.1A,H2B/a,H2BFA,H2BFL,HIST1H2BC,H2BFH,HIST1H2BE,H2BFG,HIST1H2BF,H2BFK,HIST1H2BI,H2BC4,H2BC6,H2BC7,H2BC8,H2BC10,Histone post-translational modifications (PTMs) are key mechanisms of epigenetics that modulate chromatin structures, termed as ""histone code"". The PTMs on histone including acetylation, methylation, phosphorylation and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability, gene transcription, etc. Histone acetylation occurs primarily at multiple lysine residues on the amino-terminal of core histones, in response to various stimuli and plays vital roles in the regulation of gene expression, DNA damage repair, chromatin dynamics, etc. Mostly, histone H2A is primarily acetylated at Lys5, 9, 15, and 36, H2B is primarily acetylated at Lys5, 12, 15, 16, and 20. Histone H3 is primarily acetylated at Lys4, 9, 14, 18, 23, 27, 56, and 79. Histone H4 is primarily acetylated at Lys5, 8, 12, 16, and 20. Histone acetyltransferases (HATs) and histone deacetylases (HDACs) are major regulating factors. Cat.No. Product Name Clone No. IF:{{item.impact}} Journal:{{item.journal}} ({{item.year}}) DOI:{{item.doi}} Reactivity:{{item.species}} Sample Type:{{item.sample_type}} Previous {{ page }} Next Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}} Previous {{ page }} Next [

Molecular Weight:

Calculated MW: 14 kDa

Observed MW: 15kDa The actual band is not consistent with the expectation.

UniProt: P62807