Recombinant PIM1 antibody

Catalog No. ABIN7828461

The Rabbit Monoclonal anti-PIM1 antibody (Clone A745) (ABIN7828461) specifically detects PIM1 in WB. The antibody is reactive with Human samples.

Quick Overview for Recombinant PIM1 antibody (ABIN7828461)

Target: PIM1 (Pim-1 Oncogene (PIM1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PIM1 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: A745

Product Details anti-PIM1 Antibody

Purpose: Recombinant Pim-1 Monoclonal Antibody

Purification: Protein A purified

Isotype: IgG, kappa

Application Details

Application Notes: WB 1:1000

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 % protein protectant.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Expiry Date: 12 months

Target Details for PIM1

Target: PIM1 (Pim-1 Oncogene (PIM1))

Alternative Name: Pim-1

Background: PIM,PIM1,Oncogene PIM 1,Oncogene PIM1,PIM 1,Pim 1 kinase,pim 1 kinase 44 kDa isoform,Pim 1 oncogene,pim 1 oncogene (proviral integration site 1),pim1 kinase 44 kDa isoform,Pim2,PIM3,Proto oncogene serine/threonine protein kinase Pim 1,Proto-oncogene serine/threonine-protein kinase Pim-1,Proviral integration site 1,Proviral integration site 2,Pim proteins (Pim-1, Pim-2 and Pim-3) are oncogene-encoded serine/threonine kinases. Pim-1, a serine/threonine kinase highly expressed in hematopoietic cells, plays a critical role in the transduction of mitogenic signals and is rapidly induced by a variety of growth factors and cytokines. Pim-1 cooperates with c-Myc in lymphoid cell transformation and protects cells from growth factor withdrawal and genotoxic stress-induced apoptosis. Pim-1 also enhances the transcriptional activity of c-Myb through direct phosphorylation within the c-Myb DNA binding domain as well as phosphorylation of the transcriptional coactivator p100. Hypermutations of the Pim-1 gene are found in B-cell diffuse large cell lymphomas. Phosphorylation of Pim-1 at Tyr218 by Etk occurs following IL-6 stimulation and correlates with an increase in Pim-1 activity. Various Pim substrates have been identified, Bad is phosphorylated by both Pim-1 and Pim-2 at Ser112 and this phosphorylation reverses Bad-induced cell apoptosis.The corresponding pim-1 gene encodes a pair of proteins through use of different translation initiation sites. Both larger 44 kDa (Pim-1L) and smaller 33 kDa (Pim-1S) proteins are active kinases, but differ in stability. Cat.No. Product Name Clone No. IF:{{item.impact}} Journal:{{item.journal}} ({{item.year}}) DOI:{{item.doi}} Reactivity:{{item.species}} Sample Type:{{item.sample_type}} Previous {{ page }} Next Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}} Previous {{ page }} Next [

Molecular Weight:

Calculated MW: 36 kDa

Observed MW: 33, 34 kDa The actual band is not consistent with the expectation.

UniProt: P11309

Pathways: Glycosaminoglycan Metabolic Process