Recombinant PYCARD antibody

Catalog No. ABIN7828507

This Rabbit Monoclonal antibody specifically detects PYCARD in WB, IHC and IF. It exhibits reactivity toward Human.

Quick Overview for Recombinant PYCARD antibody (ABIN7828507)

Target: PYCARD (PYD and CARD Domain Containing (PYCARD))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PYCARD antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: A619

Product Details anti-PYCARD Antibody

Purpose: Recombinant TMS1/ASC Monoclonal Antibody

Purification: Protein A purified

Immunogen: Recombinant human TMS1/ASC fragment

Isotype: IgG, kappa

Application Details

Application Notes: WB 1:1000,IHC 1:100-1:500,IF 1:50

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 % protein protectant.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Expiry Date: 12 months

Target Details for PYCARD

Target: PYCARD (PYD and CARD Domain Containing (PYCARD))

Alternative Name: TMS1/ASC

Background: CARD,ASC / TMS,PYCARD,ASC,CARD5,TMS,TMS-1,TMS1,PYD and CARD domain containing,ASC / TMS1,Apoptosis associated speck like protein containing a CARD,Apoptosis-associated speck-like protein containing a CARD,CARD 5,Caspase recruitment domain containing protein 5,Caspase recruitment domain protein 5,Caspase recruitment domain-containing protein 5,hASC,MGC10332,PYD and CARD domain containing protein,PYD and CARD domain-containing protein,Target of methylation induced silencing 1,Target of methylation-induced silencing 1,TMS 1,Target of methylation-induced silencing (TMS1)/Apoptosis-associated speck-like protein containing a CARD (ASC), also referred to as PYCARD and CARD5, is a 22- kDa pro-apoptotic protein containing an N-terminal pyrin domain (PYD) and a C-terminal caspase recruitment domain (CARD). TheASC/TMS1gene was originally found to be aberrantly methylated and silenced in breast cancer cells, and has since been found to be silenced in a number of other cancers, including ovarian cancer, glioblastoma, melanoma, gastric cancer, lung cancer, and prostate cancer. Expression of ASC/TMS1 can be induced by pro-apoptotic/inflammatory stimuli. During apoptosis ASC/TMS1 is re-distributed from the cytosol to the mitochondria and associates with mitochondrial Bax to trigger cytochrome c release and subsequent apoptosis. ASC/TMS1 has also been found to be a critical component of inflammatory signaling where it associates with and activates caspase-1 in response to pro-inflammatory signals. Cat.No. Product Name Clone No. IF:{{item.impact}} Journal:{{item.journal}} ({{item.year}}) DOI:{{item.doi}} Reactivity:{{item.species}} Sample Type:{{item.sample_type}} Previous {{ page }} Next Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}} Previous {{ page }} Next [

Molecular Weight:

Calculated MW: 22, 15 kDa

Observed MW: 22, 15 kDa

UniProt: Q9ULZ3

Pathways: Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation, Inflammasome