Recombinant SIRT1 antibody (pSer47)

Catalog No. ABIN7828566

The Rabbit Monoclonal anti-SIRT1 antibody (Clone A810) (ABIN7828566) specifically detects SIRT1 in WB and IHC. The antibody is reactive with Human samples.

Quick Overview for Recombinant SIRT1 antibody (pSer47) (ABIN7828566)

Target: SIRT1 (Sirtuin 1 (SIRT1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This SIRT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: A810

Product Details anti-SIRT1 Antibody

Binding Specificity: pSer47

Purpose: Recombinant Phospho-SIRT1(Ser47) Monoclonal Antibody

Purification: Protein A purified

Immunogen: phosphorylated human SIRT1(Ser47) peptide

Isotype: IgG, kappa

Application Details

Application Notes: WB 1:500-1:1000,IHC 1:50-1:200

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 % protein protectant.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Expiry Date: 12 months

Target Details for SIRT1

Target: SIRT1 (Sirtuin 1 (SIRT1))

Alternative Name: SIRT1

Background: SIR,HST,SIRT,Hsir,homolog-like,SIR2 like protein,Regulatory protein SIR2 homolog,NAD-dependent deacetylase sirtuin,Sirtuin (silent mating type information regulation 2 homolog),SIR2L,Hsirt,75SirT,sirtuin,SIRT1,SIR2,SIR2L1,SIR2alpha,sirtuin 1,75SirT1,Hsir2,Hsirt1,HST2,S.cerevisiae,NAD-dependent deacetylase sirtuin-1,Regulatory protein SIR2 homolog 1,SIR2 like protein 1,homolog-like 1,SirtT1 75 kDa fragment,Sirtuin (silent mating type information regulation 2 homolog) 1,SIR2,SIR2alpha,SIR2L1,sirtuin 1,homolog of,NAD dependent deacetylase sirtuin 1,NAD dependent protein deacetylase sirtuin 1,OTTHUMP00000198111,OTTHUMP00000198112,S. cerevisiae,SIR1,SIR2 like 1,SIR2-like protein 1,Sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae),Sirtuin type 1,SIRT1, the mammalian ortholog of Sir2, is a nuclear protein implicated in the regulation of many cellular processes, including apoptosis, cellular senescence, endocrine signaling, glucose homeostasis, aging, and longevity. Targets of SirT1 include acetylated p53, p300, Ku70, forkhead (FoxO) transcription factors, PPARγ, and the PPARγ coactivator-1α (PGC-1α) protein. Deacetylation of p53 and FoxO transcription factors represses apoptosis and increases cell survival. Deacetylation of PPARγ and PGC-1α regulates the gluconeogenic/glycolytic pathways in the liver and fat mobilization in white adipocytes in response to fasting. SirT1 deacetylase activity is inhibited by nicotinamide and activated by resveratrol. In addition, SirT1 activity may be regulated by phosphorylation, as it is phosphorylated at Ser27 and Ser47 in vivo, however, the function of these phosphorylation sites has not yet been determined. Cat.No. Product Name Clone No. IF:{{item.impact}} Journal:{{item.journal}} ({{item.year}}) DOI:{{item.doi}} Reactivity:{{item.species}} Sample Type:{{item.sample_type}} Previous {{ page }} Next Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}} Previous {{ page }} Next [

Molecular Weight:

Calculated MW: 82 kDa

Observed MW: 110 kDa The actual band is not consistent with the expectation.

UniProt: Q96EB6

Pathways: MAPK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Negative Regulation of intrinsic apoptotic Signaling