CRISPR-Cas9 antibody

Catalog No. ABIN7833198

This Rabbit Polyclonal antibody specifically detects CRISPR-Cas9 in WB and IL. It exhibits reactivity toward Staphylococcus aureus.

Quick Overview for CRISPR-Cas9 antibody (ABIN7833198)

Target: CRISPR-Cas9

Reactivity: Staphylococcus aureus

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunolocalization (IL)

Product Details

Purpose: Anti-Cas9 | CRISPR-associated endonuclease 9 (polyclonal)

Cross-Reactivity (Details): Staphylococcus aureus

Predicted Reactivity: Staphylococcus pyogenes UniProt: Q99ZW2

Purification: Immunogen affinity purified serum

Immunogen: KLH-conjugated synthetic peptide derived from Streptococcus thermophilus Cas9/Csn1 protein sequence

Application Details

Application Notes: 1 : 100 (IL), 1 : 1000 (WB)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Target Details

Target: CRISPR-Cas9

Alternative Name: Cas9

Background: Cas9 (CRISPR-associated endonuclease 9) is an RNA-guided DNA endonuclease associated with the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) type II adaptive immunity system. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas9 protein serves as a genome engineering tool to induce site-directed double strand breaks in DNA.

Molecular Weight: Depends on the MW of the protein that is the fusion partner.

UniProt: Q03JI6