eGFP antibody

Catalog No. ABIN7833234

This Rabbit Polyclonal antibody specifically detects eGFP in WB and ICC. It exhibits reactivity toward Aequorea victoria.

Quick Overview for eGFP antibody (ABIN7833234)

Target: eGFP (Enhanced Green Fluorescent Protein (eGFP))

Reactivity: Aequorea victoria

Host: Rabbit

Clonality: Polyclonal

Conjugate: This eGFP antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Product Details anti-eGFP Antibody

Purpose: Anti-EGFP | Enhanced Green Fluorescence Protein

Cross-Reactivity (Details): GFP, EGFP
Not reactive in: mCherry

Purification: Immunogen affinity purified

Immunogen: Recombinant GFP from Aequorea coerulescens (belt jellyfish) overexpressed and purified from E.coli

Application Details

Application Notes: 1:2000 - 1:5000 (ICC), 1:1000 - 1:5000 (WB)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μL of sterile water, ultrapure water

Buffer: Immunogen affinity purified in PBS pH 7.2-7.6, 0.1 % trehalose, 5 mM sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store lypholized antibody at 4 °C. After reconstitution keep aliquots at -20°C for a higher stability. Avoid repetitive freeze/thaw cycles. Centrifuge briefly to remove any insoluble material.Expiry date: 12 months after purchase if unopened

Expiry Date: 12 months

Target Details for eGFP

Target: eGFP (Enhanced Green Fluorescent Protein (eGFP))

Alternative Name: EGFP

Background: EGFP is an Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin. Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Xaa-N and Gly-(N+2), and oxidation of Tyr-(N+1) to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.

Molecular Weight: 27 kDa (for GFP without fusion partner)