KLH antibody (Biotin)

Catalog No. ABIN7833397

The Rabbit Polyclonal anti-KLH antibody is suitable to detect KLH in samples from Keyhole Limpet. It has been validated for ELISA, WB and IL.

Quick Overview for KLH antibody (Biotin) (ABIN7833397)

Target: KLH (Keyhole Limpet Hemocyanin (KLH))

Reactivity: Keyhole Limpet

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KLH antibody is conjugated to Biotin

Application: ELISA, Western Blotting (WB), Immunolocalization (IL)

Product Details anti-KLH Antibody (Biotin)

Purpose: Anti-KLH | Keyhole limpet hemocyanin, Biotin-conjugated

Cross-Reactivity (Details): Megathura crenulata - most commonly used carrier protein
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Purification: Immunogen affinity purified serum

Immunogen: Purified keyhole limpet hemocyanin (KLH)

Application Details

Application Notes: 1: 5 000 (ELISA), 1: 5 000 (WB), 1: 1000 (IL)

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Immunogen affinity purified serum in PBS, pH 7.4, conjugated to biotin.

Handling Advice: A preservative may be added for long time storage

Storage: 4 °C

Storage Comment: Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.

Expiry Date: 12-24 months

Target Details for KLH

Target: KLH (Keyhole Limpet Hemocyanin (KLH))

Alternative Name: KLH

Background:

Background: Keyhole limpet hemocyanin (KLH) is a large cooper-containing protein consisting of subunits with MW of 400 kDa. It is found in the hemolymph of the sea mollusk Megathura crenulata. This extracellular respiratory protein has many immunostimulatory properties, including the ability to enhance the host's immune response by interacting with T cells, monocytes, macrophages, and polymorphonuclear lymphocytes. Since its discovery, KLH has been used primarily as a carrier for vaccines and antigens and as adjuvant treatment in regimens such as antimicrobial therapy.

Additional information: Protein present in plant vascular tissue (xylem and vascular cambium) is detected by anti-KLH antibodies (Höglund et al. 2002) which might lead to false results in IL when using anti-peptide antibodies generated to KLH-conjugated peptide.

Molecular Weight: ca. 400 kDa/subunit

UniProt: Q6KC56