KLH antibody

Catalog No. ABIN7833398

The Mouse Monoclonal anti-KLH antibody (Clone 6D12) (ABIN7833398) specifically detects KLH in ELISA and WB. The antibody is reactive with Keyhole Limpet samples.

Quick Overview for KLH antibody (ABIN7833398)

Target: KLH (Keyhole Limpet Hemocyanin (KLH))

Reactivity: Keyhole Limpet

Host: Mouse

Clonality: Monoclonal

Conjugate: This KLH antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Clone: 6D12

Product Details anti-KLH Antibody

Purpose: Anti-KLH | Keyhole Limpet Hemocyanin (monoclonal, clone 6D12)

Cross-Reactivity (Details): Reacts with Keyhole Limpet 8-9 kDa Hemocyanin protein.

Immunogen: Hemocyanin from Megathura crenulata.

Isotype: IgG1, kappa

Application Details

Application Notes: 1:10 000 - 1:100 000 (ELISA), 1: 500 - 1: 5000 (WB)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in distilled water.

Buffer: Purified on Protein A to a total immunoglobulin fraction, in Tris 0.1 M, glycine 0.1 M and 2 % sucrose.

Storage: 4 °C,-20 °C

Storage Comment: Lyophilized powder is stable for a minimum of 2 years at -20°C. Reconstitute in distilled water before use. Store reconstituted antibodies at 4°C for up to a month and make aliquots for extended storage.

Expiry Date: 24 months

Target Details for KLH

Target: KLH (Keyhole Limpet Hemocyanin (KLH))

Alternative Name: KLH

Background: Keyhole limpet hemocyanin (KLH) is a large cooper-containing protein consisting of subunits with MW of 400 kDa. It is found in the hemolymph of the sea mollusk Megathura crenulata. This extracellular respiratory protein has many immunostimulatory properties, including the ability to enhance the host's immune response by interacting with T cells, monocytes, macrophages, and polymorphonuclear lymphocytes. Since its discovery, KLH has been used primarily as a carrier for vaccines and antigens and as adjuvant treatment in regimens such as antimicrobial therapy.

Molecular Weight: ca. 400 kDa/subunit

UniProt: Q6KC56