IL7R antibody (PE-Cy7)

Catalog No. ABIN7835718

This Mouse Monoclonal antibody specifically detects IL7R in FACS and IF. It exhibits reactivity toward Human.

Quick Overview for IL7R antibody (PE-Cy7) (ABIN7835718)

Target: IL7R (Interleukin 7 Receptor (IL7R))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL7R antibody is conjugated to PE-Cy7

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: A019D5

Product Details anti-IL7R Antibody (PE-Cy7)

Purpose: Mouse monoclonal antibody PE/Cy7 labled to CD127

Specificity: Recognizes human CD127

Purification: The antibody was purified by affinity chromatography.

Immunogen: Recombinant human CD127

Application Details

Application Notes: Flow: 10 μL / assay

Protocol: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Shipped and store at 4°C for one year. Do not freeze.

Target Details for IL7R

Target: IL7R (Interleukin 7 Receptor (IL7R))

Alternative Name: IL7R

Background: Interleukin-7 receptor subunit alpha, IL-7 receptor subunit alpha, IL-7R subunit alpha, IL-7R-alpha, IL-7RA, CDw127, CD127

Gene ID: 3575

UniProt: P16871

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Regulation of Cell Size