CD1a antibody

Catalog No. ABIN7835903

The Mouse Monoclonal anti-CD1a antibody (Clone HI149) (ABIN7835903) specifically detects CD1a in FACS and IF. The antibody is reactive with Human samples.

Quick Overview for CD1a antibody (ABIN7835903)

Target: CD1a

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD1a antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: HI149

Product Details anti-CD1a Antibody

Purpose: Mouse monoclonal antibody to CD1a

Specificity: Recognizes human CD1a

Purification: The antibody was purified by affinity chromatography.

Immunogen: Native purified human CD1a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Target Details for CD1a

Target: CD1a

Alternative Name: CD1A

Background: T-cell surface glycoprotein CD1a, T-cell surface antigen T6/Leu-6, hTa1 thymocyte antigen, CD1a

Gene ID: 909

UniProt: P06126

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process