CD200 antibody (APC)

Catalog No. ABIN7835936

The APC-conjugated Mouse Monoclonal anti-CD200 antibody (Clone OX-104) (ABIN7835936) specifically detects CD200 in FACS and IF. The antibody is reactive with Human samples.

Quick Overview for CD200 antibody (APC) (ABIN7835936)

Target: CD200

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD200 antibody is conjugated to APC

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: OX-104

Product Details anti-CD200 Antibody (APC)

Purpose: Mouse monoclonal antibody APC labled to CD200

Specificity: Recognizes human CD200

Purification: The antibody was purified by affinity chromatography.

Application Details

Application Notes: Flow: 10 μL / assay

Protocol: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Shipped and store at 4°C for one year. Do not freeze.

Target Details for CD200

Target: CD200

Alternative Name: CD200

Background: MOX1, MOX2, OX-2 membrane glycoprotein, CD200

Gene ID: 4345

UniProt: P41217