ITGB1 antibody (PE)

Catalog No. ABIN7836089

The PE-conjugated Mouse Monoclonal anti-ITGB1 antibody (Clone TS2-16-2-1) (ABIN7836089) specifically detects ITGB1 in FACS and IF. The antibody is reactive with Human samples.

Quick Overview for ITGB1 antibody (PE) (ABIN7836089)

Target: ITGB1 (Integrin beta 1 (ITGB1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ITGB1 antibody is conjugated to PE

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: TS2-16-2-1

Product Details anti-ITGB1 Antibody (PE)

Purpose: Mouse monoclonal antibody PE labled to CD29

Specificity: Recognizes human CD29

Purification: The antibody was purified by affinity chromatography.

Immunogen: Native purified human CD29.

Application Details

Application Notes: Flow: 10 μL / assay

Protocol: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Shipped and store at 4°C for one year. Do not freeze.

Target Details for ITGB1

Target: ITGB1 (Integrin beta 1 (ITGB1))

Alternative Name: ITGB1

Background: FNRB, MDF2, MSK12, Integrin beta-1, Fibronectin receptor subunit beta, Glycoprotein IIa, GPIIA, VLA-4 subunit beta, CD antigen CD29

Gene ID: 3688

UniProt: P05556

Pathways: Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor, Integrin Complex, SARS-CoV-2 Protein Interactome