ITGA4 antibody (APC)

Catalog No. ABIN7836415

The Mouse Monoclonal anti-ITGA4 antibody is suitable to detect ITGA4 in samples from Human. It has been validated for FACS and IF.

Quick Overview for ITGA4 antibody (APC) (ABIN7836415)

Target: ITGA4 (Integrin alpha 4 (ITGA4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ITGA4 antibody is conjugated to APC

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: BU49

Product Details anti-ITGA4 Antibody (APC)

Purpose: Mouse monoclonal antibody APC labled to CD49d

Specificity: Recognizes human CD49d

Purification: The antibody was purified by affinity chromatography.

Immunogen: Native purified human CD49d.

Application Details

Application Notes: Flow: 10 μL / assay

Protocol: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Shipped and store at 4°C for one year. Do not freeze.

Target Details for ITGA4

Target: ITGA4 (Integrin alpha 4 (ITGA4))

Alternative Name: ITGA4

Background: CD49D, Integrin alpha-4, CD49 antigen-like family member D, Integrin alpha-IV, VLA-4 subunit alpha, CD antigen CD49d

Gene ID: 3676

UniProt: P13612

Pathways: Integrin Complex