CD72 antibody (PE)

Catalog No. ABIN7836577

The Mouse Monoclonal anti-CD72 antibody is suitable to detect CD72 in samples from Mouse. It has been validated for FACS and IF.

Quick Overview for CD72 antibody (PE) (ABIN7836577)

Target: CD72 (CD72 Molecule (CD72))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD72 antibody is conjugated to PE

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 10-1-D2

Product Details anti-CD72 Antibody (PE)

Purpose: Mouse monoclonal antibody PE labled to CD72.1

Specificity: Recognizes mouse CD72.1

Purification: The antibody was purified by affinity chromatography.

Immunogen: DBA/2 mouse spleen cells

Application Details

Application Notes: Flow: 10 μL / assay

Protocol: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Shipped and store at 4°C for one year. Do not freeze.

Target Details for CD72

Target: CD72 (CD72 Molecule (CD72))

Alternative Name: CD72

Background: B-cell differentiation antigen CD72, Lyb-2, CD72

Gene ID: 12517

Pathways: BCR Signaling