CD8B antibody

Catalog No. ABIN7836671

The Rat Monoclonal anti-CD8B antibody (Clone 53-5-8) (ABIN7836671) specifically detects CD8B in FACS and IF. The antibody is reactive with Mouse samples.

Quick Overview for CD8B antibody (ABIN7836671)

Target: CD8B (CD8b Molecule (CD8B))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD8B antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 53-5-8

Product Details anti-CD8B Antibody

Purpose: Rat monoclonal antibody to CD8b.2

Specificity: Recognizes mouse CD8b.2

Purification: The antibody was purified by affinity chromatography.

Immunogen: Mouse thymus or spleen

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Target Details for CD8B

Target: CD8B (CD8b Molecule (CD8B))

Alternative Name: CD8B

Background: CD8B1, T-cell surface glycoprotein CD8 beta chain, CD8b

Gene ID: 12526

Pathways: TCR Signaling