CD99 antibody (PE)

Catalog No. ABIN7836716

The PE-conjugated Mouse Monoclonal anti-CD99 antibody (Clone 3B2-TA8) (ABIN7836716) specifically detects CD99 in FACS and IF. The antibody is reactive with Human samples.

Quick Overview for CD99 antibody (PE) (ABIN7836716)

Target: CD99

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD99 antibody is conjugated to PE

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 3B2-TA8

Product Details anti-CD99 Antibody (PE)

Purpose: Mouse monoclonal antibody PE labled to CD99

Specificity: Recognizes human CD99

Purification: The antibody was purified by affinity chromatography.

Application Details

Application Notes: Flow: 10 μL / assay

Protocol: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Shipped and store at 4°C for one year. Do not freeze.

Target Details for CD99

Target: CD99

Alternative Name: CD99

Background: MIC2, MIC2X, MIC2Y, CD99 antigen, 12E7, E2 antigen, Protein MIC2, T-cell surface glycoprotein E2, CD99

Gene ID: 4267

UniProt: P14209