Hepsin antibody

Catalog No. ABIN7839442

The Mouse Monoclonal anti-Hepsin antibody is suitable to detect Hepsin in samples from Human. It has been validated for IF and FACS.

Quick Overview for Hepsin antibody (ABIN7839442)

Target: Hepsin (HPN)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Hepsin antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: 2D5

Product Details anti-Hepsin Antibody

Purpose: Mouse monoclonal antibody to Hepsin

Specificity: Recognizes human Hepsin

Purification: The antibody was purified by affinity chromatography.

Immunogen: Recombinant fusion protein of human HPN

Application Details

Application Notes: IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)

Protocol: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Target Details for Hepsin

Target: Hepsin (HPN)

Alternative Name: HPN

Background: TMPRSS1, Serine protease hepsin, Transmembrane protease serine 1

Gene ID: 3249

UniProt: P05981

Pathways: Regulation of Hormone Metabolic Process, Sensory Perception of Sound