HLA-A antibody

Catalog No. ABIN7839650

The Mouse Monoclonal anti-HLA-A antibody (Clone W6132) (ABIN7839650) specifically detects HLA-A in FACS and IF. The antibody is reactive with Human samples.

Quick Overview for HLA-A antibody (ABIN7839650)

Target: HLA-A (Major Histocompatibility Complex, Class I, A (HLA-A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HLA-A antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: W6132

Product Details anti-HLA-A Antibody

Purpose: Mouse monoclonal antibody to HLA-A/B/C

Specificity: Recognizes human HLA-A/B/C

Purification: The antibody was purified by affinity chromatography.

Application Details

Application Notes: IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)

Protocol: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Target Details for HLA-A

Target: HLA-A (Major Histocompatibility Complex, Class I, A (HLA-A))

Alternative Name: HLA-A

Background: HLA-A, HLAA, HLA class I histocompatibility antigen, A-68 alpha chain, Aw-68, HLA class I histocompatibility antigen, A-28 alpha chain, MHC class I antigen A*68, HLA-A, HLAA, HLA class I histocompatibility antigen, A-2 alpha chain, MHC class I antigen A*2, HLA-A, HLAA, HLA class I histocompatibility antigen, A-3 alpha chain, MHC class I antigen A*3, HLA-A, HLAA, HLA class I histocompatibility antigen, A-24 alpha chain, Aw-24, HLA class I histocompatibility antigen, A-9 alpha chain, MHC class I antigen A*24, HLA-A, HLAA, HLA class I histocompatibility antigen, A-32 alpha chain, MHC class I antigen A*32, HLA-A, HLAA, HLA class I histocompatibility antigen, A-69 alpha chain, Aw-69, HLA class I histocompatibility antigen, A-28 alpha chain, MHC class I antigen A*69, HLA-A, HLAA, HLA class I histocompatibility antigen, A-11 alpha chain, MHC class I antigen A*11, HLA-A, HLAA, HLA class I histocompatibility antigen, A-31 alpha chain, MHC class I antigen A*31, HLA-A, HLAA, HLA class I histocompatibility antigen, A-25 alpha chain, HLA class I histocompatibility antigen, A-10 alpha chain, MHC class I antigen A*25, HLA-A, HLAA, HLA class I histocompatibility antigen, A-1 alpha chain, MHC class I antigen A*1, HLA-A, HLAA, HLA class I histocompatibility antigen, A-26 alpha chain, MHC class I antigen A*26, HLA-A, HLAA, HLA class I histocompatibility antigen, A-34 alpha chain, Aw-34, HLA class I histocompatibility antigen, A-10 alpha chain, MHC class I antigen A*34, HLA-A, HLAA, HLA class I histocompatibility antigen, A-36 alpha chain, Aw-36, MHC class I antigen A*36

Gene ID: 3105

UniProt: P04439

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints, Human Leukocyte Antigen (HLA) in Adaptive Immune Response