HLA-DRA antibody (APC)

Catalog No. ABIN7839674

The APC-conjugated Mouse Monoclonal anti-HLA-DRA antibody (Clone LN3) (ABIN7839674) specifically detects HLA-DRA in FACS and IF. The antibody is reactive with Human samples.

Quick Overview for HLA-DRA antibody (APC) (ABIN7839674)

Target: HLA-DRA (HLA Class II DR alpha (HLA-DRA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HLA-DRA antibody is conjugated to APC

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: LN3

Product Details anti-HLA-DRA Antibody (APC)

Purpose: Mouse monoclonal antibody APC labled to HLA-DR

Specificity: Recognizes human HLA-DR

Purification: The antibody was purified by affinity chromatography.

Immunogen: Native purified human HLA-DR.

Application Details

Application Notes: Flow: 10 μL / assay

Protocol: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Shipped and store at 4°C for one year. Do not freeze.

Target Details for HLA-DRA

Target: HLA-DRA (HLA Class II DR alpha (HLA-DRA))

Alternative Name: HLA-DRA

Background: HLA-DRA1, HLA class II histocompatibility antigen. DR alpha chain, MHC class II antigen DRA

Gene ID: 3122

UniProt: P01903

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Human Leukocyte Antigen (HLA) in Adaptive Immune Response