IL1A antibody

Catalog No. ABIN7840001

The Armenian Hamster Monoclonal anti-IL1A antibody (Clone ALF-161) (ABIN7840001) specifically detects IL1A in FACS and IF. The antibody is reactive with Mouse samples.

Quick Overview for IL1A antibody (ABIN7840001)

Target: IL1A (Interleukin 1 alpha (IL1A))

Reactivity: Mouse

Host: Armenian Hamster

Clonality: Monoclonal

Conjugate: This IL1A antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: ALF-161

Product Details anti-IL1A Antibody

Purpose: Armenian Hamster monoclonal antibody to IL-1 alpha

Specificity: Recognizes mouse IL-1 alpha

Purification: The antibody was purified by affinity chromatography.

Immunogen: E. coli - expressed, recombinant mouse IL-1

Application Details

Application Notes: IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)

Protocol: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Target Details for IL1A

Target: IL1A (Interleukin 1 alpha (IL1A))

Alternative Name: IL1A

Background: IL1F1, Interleukin-1 alpha, IL-1 alpha, Hematopoietin-1

Gene ID: 16175

Pathways: NF-kappaB Signaling, Autophagy, Cancer Immune Checkpoints