IL12B antibody

Catalog No. ABIN7840010

The Mouse Monoclonal anti-IL12B antibody (Clone ABT-874) (ABIN7840010) specifically detects IL12B in FACS and IF. The antibody is reactive with Human samples.

Quick Overview for IL12B antibody (ABIN7840010)

Target: IL12B (Interleukin 12 beta (IL12B))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL12B antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: ABT-874

Product Details anti-IL12B Antibody

Purpose: Mouse monoclonal antibody to IL-12 p40

Specificity: Recognizes human IL-12 p40

Purification: The antibody was purified by affinity chromatography.

Immunogen: Recombinant human IL-12 (p40)

Application Details

Application Notes: IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)

Protocol: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Target Details for IL12B

Target: IL12B (Interleukin 12 beta (IL12B))

Alternative Name: IL12B

Background: NKSF2, Interleukin-12 subunit beta, IL-12B, Cytotoxic lymphocyte maturation factor 40 kDa subunit, CLMF p40, IL-12 subunit p40, NK cell stimulatory factor chain 2, NKSF2

Gene ID: 3593

UniProt: P29460

Pathways: JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation