Ki-67 antibody (FITC)

Catalog No. ABIN7840347

This Mouse Monoclonal antibody specifically detects Ki-67 in FACS and IF. It exhibits reactivity toward Human.

Quick Overview for Ki-67 antibody (FITC) (ABIN7840347)

Target: Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Ki-67 antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 3D5

Product Details anti-Ki-67 Antibody (FITC)

Purpose: Mouse monoclonal antibody FITC labled to Ki67

Specificity: Recognizes human Ki67

Purification: The antibody was purified by affinity chromatography.

Immunogen: Recombinant fusion protein of human Ki67.

Application Details

Application Notes: Flow: 10 μL / assay

Protocol: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Shipped and store at 4°C for one year. Do not freeze.

Target Details for Ki-67

Target: Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

Alternative Name: MKI67

Background: Antigen KI-67

Gene ID: 4288

UniProt: P46013

Pathways: Glycosaminoglycan Metabolic Process