SREBF2 antibody (HRP)

Catalog No. ABIN741714

The HRP-conjugated Rabbit Polyclonal anti-SREBF2 antibody (ABIN741714) specifically detects SREBF2 in IHC (p) and IHC (fro). The antibody is reactive with Human samples.

Quick Overview for SREBF2 antibody (HRP) (ABIN741714)

Target: SREBF2 (Sterol Regulatory Element Binding Transcription Factor 2 (SREBF2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SREBF2 antibody is conjugated to HRP

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-SREBF2 Antibody (HRP)

Purpose: SREBP2 Polyclonal Antibody, HRP Conjugated

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human SREBP-2

Isotype: IgG

Application Details

Application Notes: IHC-P(1:200-400), IHC-F(1:100-500)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for SREBF2

Target: SREBF2 (Sterol Regulatory Element Binding Transcription Factor 2 (SREBF2))

Alternative Name: SREBP-2

Background:

Synonyms: Sterol Regulatory Element Binding Protein-2, MGC124823, Srebf2_retired, SREBP-2, SREBP2, SRBP2_HUMAN, Sterol regulatory element-binding protein 2, SREBP-2, Class D basic helix-loop-helix protein 2, bHLHd2, Sterol regulatory element-binding transcription factor 2, Processed sterol regulatory element-binding protein 2.

Background: Under basal conditions SREBP is bound to ER membranes as a glycosylated precursor protein. Upon cholesterol depletion, the protein is cleaved to its active forms (50-68 kDa) and translocated into the nucleus to stimulate transcription of genes involved in the uptake and synthesis of cholesterol. ab30682 detects both precursor and active forms of SREBP2 in tissues and cells such as liver, brown fat, testis, HepG2 cells, and human fibroblast. The apparent molecular weight on SDS-PAGE may be higher than the calculated molecular weight (about 126 kDa) due to glycosylation of the protein.

Molecular Weight: 126kDa

Gene ID: 6721

Pathways: Regulation of Lipid Metabolism by PPARalpha