Hemagglutinin antibody

Catalog No. ABIN7849481

Supplier Product No.: 200-301-nh4

The Mouse Monoclonal anti-Hemagglutinin antibody is suitable to detect Hemagglutinin in samples from Influenza A Virus H5N1. It has been validated for ELISA, WB, HI, BLI, VNT and InhA.

Quick Overview for Hemagglutinin antibody (ABIN7849481)

Target: Hemagglutinin (HA)

Reactivity: Influenza A Virus H5N1

Host: Mouse

Clonality: Monoclonal

Conjugate: This Hemagglutinin antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Haemagglutination Inhibition (HI), Bio-Layer Interferometry (BLI), Virus Neuralisation Test (VNT), Inhibition Assay (InhA)

Clone: 1D4-G1

Product Details anti-Hemagglutinin Antibody

Virus Strain: H5N1, clade 2.3.4.4b

Supplier Product No.: 200-301-nh4

Supplier: Rockland

Purpose: Monoclonal antibody raised against recombinant hemagglutinin (HA) from H5N1 clade 2.3.4.4b. (1D4)

Specificity: This monoclonal antibody is part of a targeted panel generated against the recombinant HA ectodomain from H5N1 clade 2.3.4.4b, supporting studies of antigenicity, viral evolution, and immune recognition.

Characteristics: mouse anti-H5 Antibody, mouse anti-Avian influenza A Antibody, Hemagglutinin antibody, Hemagglutinin 5 antibody, H5N1

Purification: This product was purified from tissue culture supernatant fluid by Protein A chromatography and is specific for Avian influenza A (H5N1) clade 2.

Sterility: Sterile filtered

Immunogen:

Immunogen: This protein A purified monoclonal antibody was produced by intraperitoneal immunization of BALB/c mice with concentrated purified virus preparation containing hemagglutinin (HA) protein of influenza A virus [clade 2.3.4.4b].

Immunogen Type: Recombinant Protein

Isotype: IgG1, kappa

Application Details

Application Notes: Anti-Hemagglutinin (HA) antibody has been tested in ELISA, biolayer interferometry (BLI), and HI assays with 1% horse RBC.  This antibody may be used for hemagglutination inhibition (HI) assays to provide antigenic characterization of the influenza A viruses of the H5 HA subtype and may be suitable for virus neutralization assays (in cell culture and in embryonated chicken eggs), ELISA, immunoprecipitation, immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.01 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use

Storage: -20 °C

Storage Comment: Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Hemagglutinin

Target: Hemagglutinin (HA)

Alternative Name: HA

Target Type: Influenza Protein

Background: Highly pathogenic avian influenza (HPAI) H5N1 viruses of clade 2.3.4.4b have rapidly expanded in the United States since late 2021, driving unprecedented outbreaks in poultry and wild birds. HA-the primary viral surface glycoprotein-is a critical target for neutralizing antibodies, yet its high antigenic variability underscores the need for well-defined research reagents.