Caldesmon antibody (C-Term)

Catalog No. ABIN7849506

This Rabbit Monoclonal antibody specifically detects Caldesmon in WB, IHC, IF, FACS, IHC (p) and ICC. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for Caldesmon antibody (C-Term) (ABIN7849506)

Target: Caldesmon (CALD1) (Caldesmon 1 (CALD1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Caldesmon antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-Caldesmon Antibody

Binding Specificity: C-Term

Purpose: Monoclonal Anti- Caldesmon antibody

Purification: Protein A affinity purified

Immunogen: Asynthetic peptide within C-terminal human Caldesmon.

Isotype: IgG

Application Details

Application Notes: Western Blot, WB(1:500-1:1, 000), Immunohistochemistry, IHC-P (1:20-1:100), Immunocytochemistry, ICC/IF (1:20-1:100), Flow Cytometry, Flow-Cyt (1:20-1:50),

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 1*PBS ( pH 7.4), 0.2 % BSA, 40 % Glycerol and 0.05 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C after thawing (1 week). Aliquot and store at -20°C for long term (at least one year). Avoid repeated freeze and thaw cycles.

Expiry Date: 12 months

Target Details for Caldesmon

Target: Caldesmon (CALD1) (Caldesmon 1 (CALD1))

Alternative Name: Caldesmon

Background: Caldesmon is a protein that in humans is encoded by the CALD1 gene.Caldesmon (CDM) is a potential actomyosin regulatory protein found in smooth muscle and nonmuscle cells. Domain mapping and physical studies suggest that CDM is an elongated molecule with an N-terminal myosin/calmodulin-binding domain and a C-terminal tropomyosin/actin/calmodulin-binding domain separated by a 40-nm-long central helix.1The high molecular weight caldesmon (h-CaD) is predominantly expressed in smooth muscles, whereas the low molecular weight caldesmon (l-CaD) is widely distributed in nonmuscle tissues and cells.2The conserved domain of this protein possesses the binding activities to Ca++-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca++-dependent inhibition of smooth muscle contraction.

Pathways: Myometrial Relaxation and Contraction