CYBB antibody

Catalog No. ABIN7855525

The Mouse Monoclonal anti-CYBB antibody is suitable to detect CYBB in samples from Human. It has been validated for WB, IHC, IF and ICC.

Quick Overview for CYBB antibody (ABIN7855525)

Target: CYBB (Cytochrome B-245, beta Polypeptide (CYBB))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CYBB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Grade: Carrier-free

Clone: 54-1

Product Details anti-CYBB Antibody

Purpose: NOX2/gp91phox Monoclonal Antibody [54.1] - BSA/Azide-Free

Purification: Protein G

Immunogen: Partially Purified human neutrophil flavocytochrome b (heparin Ultrogellectin affinity purification in Triton X-100)

Isotype: IgG1

Application Details

Application Notes: Optimal antibody concentration should be determined by titration, however as a guideline try,WB: Use at an assay dependent dilution. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).ICC/IF: Use a concentration of 1 μg/mL.IHC-P: Use a concentration of 5 μg/mL. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol

Comment:

Myeloma/fusion partners:SP2/0

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Purified antibody in PBS. Does not contain sodium azide or BSA

Preservative: Azide free

Target Details for CYBB

Target: CYBB (Cytochrome B-245, beta Polypeptide (CYBB))

Alternative Name: NOX2/gp91phox

Background: CGD91-phox antibody,CYBB antibody,Cytochrome b(558) beta chain antibody,Cytochrome b-245 heavychain antibody,gp91-1 antibody,gp91-phox antibody,Heme-binding membrane glycoprotein gp91phox antibody,NADPHoxidase 2 antibody,Neutrophil cytochrome b 91 kDa polypeptide antibody,NOX2 antibody,p22 phagocyte B-cytochromeantibody,Superoxide-generating NADPH oxidase heavy chain subunit antibody

Gene ID: 1536

UniProt: P04839