Recombinant MBP antibody (AA 1-157)

Catalog No. ABIN7864834

The Mouse Monoclonal anti-MBP antibody (Clone rMBP-9301) (ABIN7864834) specifically detects MBP in WB and IHC. The antibody is reactive with Human samples.

Quick Overview for Recombinant MBP antibody (AA 1-157) (ABIN7864834)

Target: MBP (Myelin Basic Protein (MBP))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MBP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: RMBP-9301

Product Details anti-MBP Antibody

Binding Specificity: AA 1-157

Expression System: CHO Cells

Purpose: Recombinant Myelin Basic Protein Antibody

Specificity: Myelin basic protein (MBP) is the second most abundant protein in central nervous system (CNS) myelin: it comprises 30 % of the total protein and about 10 % of the dry weight of myelin. It is the only structural protein found so far to be essential for formation of CNS myelin, and has been called the executive molecule of myelin . MBP can interact with a number of polyanionic proteins including actin, tubulin, calmodulin, and clathrin, and negatively charged lipids, and acquires structure on binding to them. It may act as a membrane actin-binding protein, which might allow it to participate in transmission of extracellular signals to the cytoskeleton in oligodendrocytes and tight junctions in myelin. MBP may be applicable as a marker for oligodendrogliomas. rMBP/9301 is used in clinical diagnostics to detect MBP levels or myelination in human MBP. rMBP/9301 is used in a two-site ELISA with clone MBP/4273. Both are used interchangeably as capture and detection antibody.

Purification: 200ug/ml of Ab produced in CHO cell mammalian-based expression system.

Immunogen: Recombinant fragment (around aa1-157) of human MBP protein (exact sequence is proprietary)

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for MBP

Target: MBP (Myelin Basic Protein (MBP))

Alternative Name: Myelin Basic Protein

Background:

GDB, Golli MBP, myelin basic protein, Hemopoietic MBP, HMBPR, HUGO, MLD, Myelin A1 Protein, basic, Myelin Deficient, Myelin membrane encephalitogenic protein, SHI, Shiverer, SP

Human brain or astrocytoma tissue.

Molecular Weight: 13-21kDa

Gene ID: 4155, 551713

UniProt: P02686