Recombinant FAS antibody (C-Term)

Catalog No. ABIN7865340

This Mouse Monoclonal antibody specifically detects FAS in IHC. It exhibits reactivity toward Human.

Quick Overview for Recombinant FAS antibody (C-Term) (ABIN7865340)

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FAS antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: RGM30

Product Details anti-FAS Antibody

Binding Specificity: C-Term

Expression System: CHO Cells

Purpose: Recombinant CD95 / FAS / TNFRSF6 (Marker of Cancer Associated Fibroblasts) Antibody

Specificity: Fas, also known as CD95 antigen, is a 48 kD transmembrane glycoprotein. It is a member of the nerve growth factor receptor/tumor necrosis factor superfamily. This cell surface molecule mediates receptor-triggered apoptosis (programmed cell death). During embryonic and postembryonic development, many cells die by means of programmed cell death, which plays a major role in determining morphological and functional maturity in a variety of systems, including the formation of the neural network and clonal deletion of autoreactive T cells. Apoptosis is accompanied by condensation of the cytoplasm, loss of plasma membrane microvilli and extensive degradation of chromosomal DNA into oligomers of about 180 base pairs. The Fas antigen is expressed on the surface of various cell types, including activated T and B lymphocytes and T lymphoblastoid cell lines.

Purification: 200ug/ml of Ab produced in CHO cell mammalian-based expression system.

Immunogen: Prokaryotic recombinant fusion protein corresponding to the internal domain near the C-terminal end of the human Fas molecule

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for FAS

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Alternative Name: CD95

Background:

ALPS1A, Apoptosis APO1 antigen 1, Apoptosis-mediating surface antigen FAS, APT1, Delta Fas, AMA, Fas cell surface death receptor, FAS1, FASL receptor, FASLG receptor, FASTM, Surface antigen APO1, Tumor necrosis factor receptor superfamily member 6 (TNFRSF6)

MCF-7 cells. Human hepatocellular or bladder carcinoma.

Molecular Weight: 38-50kDa

Gene ID: 355, 244139

UniProt: P25445

Pathways: p53 Signaling, Apoptosis, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity